說明
Identifying their diet resources is key to understanding how a high diversity of herbivore species can co-exist without competition, as well as in terms of predicting their impacts on plant species and communities. To date describing the diets of herbivores has been slow, cumbersome and probably biased. The use of DNA metabarcoding of diet samples however overcomes these limits, but is dependent on the availability of a comprehensive reference collection of DNA sequenced material for the potentially consumed plant species. To date no such reference collection exists for Subtropical Thicket in the Eastern Cape. This project therefore aimed to establish such a reference collection, for the plant community in the Addo Elephant National Park (AENP). The data set represents the plant voucher specimens collected from two localities, their identities, and DNA sequences. The voucher specimens have been deposited in the Ria Olivier Herbarium at NMMU.
資料紀錄
此資源出現紀錄的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 357 筆紀錄。
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下載最新版本的 Darwin Core Archive (DwC-A) 資源，或資源詮釋資料的 EML 或 RTF 文字檔。
版本
以下的表格只顯示可公開存取資源的已發布版本。
如何引用
研究者應依照以下指示引用此資源。:
Kerley G (2019): FBIP: DNA barcode reference library for plants from Addo Elephant National Park for herbivore diet studies. v1.0. South African National Biodiversity Institute. Dataset/Occurrence. http://ipt.sanbi.org.za/iptsanbi/resource?r=dna_barcoding&v=1.0
權利
研究者應尊重以下權利聲明。:
此資料的發布者及權利單位為 South African National Biodiversity Institute。 This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
GBIF 註冊
此資源已向GBIF註冊，並指定以下之GBIF UUID: 2dd5d605-ce02-4496-bbba-b4723a3709c0。 South African National Biodiversity Institute 發佈此資源，並經由South African Biodiversity Information Facility同意向GBIF註冊成為資料發佈者。
關鍵字
Addo Elephant National Park; herbivore diet reference collection for DNA barcoding; Specimen
聯絡資訊
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地理涵蓋範圍
Addo Elephant National Park, two localities: Main Camp and Colchester
| 界定座標範圍 | 緯度南界 經度西界 [-33.631, 25.703], 緯度北界 經度東界 [-33.442, 25.917] |
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分類群涵蓋範圍
Most specimen have been identified to Genus level while others to Species and Subspecies level.
| Kingdom | Plantae |
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時間涵蓋範圍
| 起始日期 | 2014-01-01 |
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計畫資料
Identifying their diet resources is key to understanding how a high diversity of herbivore species can co-exist without competition, as well as in terms of predicting their impacts on plant species and communities. To date describing the diets of herbivores has been slow, cumbersome and probably biased. The use of DNA metabarcoding of diet samples however overcomes these limits, but is dependent on the availability of a comprehensive reference collection of DNA sequenced material for the potentially consumed plant species. To date no such reference collection exists for Subtropical Thicket in the Eastern Cape. This project therefore aimed to establish such a reference collection, for the plant community in the Addo Elephant National Park (AENP). The data set represents the plant voucher specimens collected from two localities, their identities, and DNA sequences. The voucher specimens have been deposited in the Ria Olivier Herbarium at NMMU.
| 計畫名稱 | DNA barcode reference library for plants from Addo Elephant National Park for herbivore diet studies |
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| 辨識碼 | IBIP-BS13100452303 |
| 經費來源 | Foundational Biodiversity Information Programme |
| 研究區域描述 | Addo Elephant National Park, two localities: Main Camp and Colchester |
參與計畫的人員:
取樣方法
Data from specimen labels / datasheets. DNA barcoding done by Prof P Taberlet of Centre National de la Recherche Scientifique, France. Specimens vouchered in Ria Olivier Herbarium, identified by PhD student (C. Weatherall-Thomas), Prof E. Campbell. Specimens that could not be identified based on morphology were barcoded and barcodes used to obtain identifications
| 研究範圍 | Addo Elephant National Park, two localities: Main Camp and Colchester |
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方法步驟描述:
- This survey will be undertaken throughout the Addo Main Camp and Colchester Sections of the Addo Elephant National Park, this to cover 8 vegetation types that occur here. An initial literature and expert-based survey indicates that approximately 500 plant species should be sampled, these reflecting both dominant plant species, plant species of conservation significance and plants that are potentially consumed by the herbivores (the latter comprising 13 species) Sampling will comprise the collection of tissue and herbarium samples. Samples will be located in the field by local experts, tissues samples stored in labelled, silica-dried tubes and matched plant species will be prepared for herbarium curation in the Ria Olivier Herbarium at the Nelson Mandela Metropolitan University. This phase (plant collection, identification and curation) will be undertaken by a team of botanists and ecologists, including Profs Campbell and Kerley, Dr Landman together with postgraduate and undergraduate students. SANParks Regional Ecologist A Gaylard will also assist with this phase, together with SANParks Field Rangers (for protection of researchers). The field work will be initiated in January 2014 (or as soon as funds are released) and will run for 12 months. In this period there will be at least three major sampling trips to cover the phenological patterns of the plant communities. The tissue samples will then be sent to France, under a BABS permit, for analysis. The DNA barcoding itself will be supported by a separate project and will be undertaken in collaboration with Dr Pierre Taberlet of the Joseph Fourier University, France. The DNA analysis will be divided into three sub-tasks: (i) DNA extraction, (ii) DNA amplification, and (iii) DNA sequencing. Sequence reads will be analysed using tools specifically designed for such analyses, including http://www.grenoble.prabi.fr/trac/OBITools. It is anticipated that these DNA analyses will require an additional 6 months.